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gp2 293 packaging cell lines  (TaKaRa)


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    Structured Review

    TaKaRa gp2 293 packaging cell lines
    Gp2 293 Packaging Cell Lines, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gp2+293+packaging+cell+line/pm38207015-261-18-23
    Average 86 stars, based on 1 article reviews
    gp2 293 packaging cell lines - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Produced:

    Article Title: Extended DNA-binding interfaces beyond the canonical SAP domain contribute to the function of replication stress regulator SDE2 at DNA replication forks
    Article Snippet: The retroviral plasmid pRetroX-TetOne puro was acquired from Clontech and amplified using NEB stable competent E. coli (high efficiency). siRNA-1 resistant SDE2 WT or ΔSAP (deletion of amino acids 395–451 along with ΔUBL 1–77) were subcloned into pRetroX-TetOne puro vector. .. Retroviruses were produced using the GP2-293 packaging cell line (Clontech), where pRetroX-TetOne puro empty vector, SDE2 WT, or ΔSAP constructs were cotransfected with the envelope vector pCMV-VSV-G, using Xfect transfection reagent (Clontech). .. U2OS cells were transduced for 16 h using 8 μg/ml polybrene (Sigma–Aldrich).

    Construct:

    Article Title: Extended DNA-binding interfaces beyond the canonical SAP domain contribute to the function of replication stress regulator SDE2 at DNA replication forks
    Article Snippet: The retroviral plasmid pRetroX-TetOne puro was acquired from Clontech and amplified using NEB stable competent E. coli (high efficiency). siRNA-1 resistant SDE2 WT or ΔSAP (deletion of amino acids 395–451 along with ΔUBL 1–77) were subcloned into pRetroX-TetOne puro vector. .. Retroviruses were produced using the GP2-293 packaging cell line (Clontech), where pRetroX-TetOne puro empty vector, SDE2 WT, or ΔSAP constructs were cotransfected with the envelope vector pCMV-VSV-G, using Xfect transfection reagent (Clontech). .. U2OS cells were transduced for 16 h using 8 μg/ml polybrene (Sigma–Aldrich).

    Transfection:

    Article Title: Extended DNA-binding interfaces beyond the canonical SAP domain contribute to the function of replication stress regulator SDE2 at DNA replication forks
    Article Snippet: The retroviral plasmid pRetroX-TetOne puro was acquired from Clontech and amplified using NEB stable competent E. coli (high efficiency). siRNA-1 resistant SDE2 WT or ΔSAP (deletion of amino acids 395–451 along with ΔUBL 1–77) were subcloned into pRetroX-TetOne puro vector. .. Retroviruses were produced using the GP2-293 packaging cell line (Clontech), where pRetroX-TetOne puro empty vector, SDE2 WT, or ΔSAP constructs were cotransfected with the envelope vector pCMV-VSV-G, using Xfect transfection reagent (Clontech). .. U2OS cells were transduced for 16 h using 8 μg/ml polybrene (Sigma–Aldrich).

    Article Title: Immunocompetent cell and expression vector expressing regulatory factors of immune function
    Article Snippet: Also, the anti-FITC CAR DNA fragment was cloned into the pMSGV retrovirus expression vector to generate a pMSGV vector free from IL-7 and CCL19 as a control (control vector (1)). (Generation of Retrovirus Introduced with IL-7×CCL19 Expression Vector) For the transduction of mouse T cells, retrovirus was generated. .. A GP2-293 packaging cell line (manufactured by Takara Bio Inc.) was transfected with the aforementioned IL-7×CCL19 expression vector (1) or control vector (1) and a pCL-Eco plasmid (manufactured by Imgenex Corp.) using Lipofectamine 2000 or 3000 (manufactured by Life Technologies Corp.) to generate retrovirus introduced with the IL-7×CCL19 expression vector (1) or the control vector (1). ..

    Expressing:

    Article Title: Immunocompetent cell and expression vector expressing regulatory factors of immune function
    Article Snippet: Also, the anti-FITC CAR DNA fragment was cloned into the pMSGV retrovirus expression vector to generate a pMSGV vector free from IL-7 and CCL19 as a control (control vector (1)). (Generation of Retrovirus Introduced with IL-7×CCL19 Expression Vector) For the transduction of mouse T cells, retrovirus was generated. .. A GP2-293 packaging cell line (manufactured by Takara Bio Inc.) was transfected with the aforementioned IL-7×CCL19 expression vector (1) or control vector (1) and a pCL-Eco plasmid (manufactured by Imgenex Corp.) using Lipofectamine 2000 or 3000 (manufactured by Life Technologies Corp.) to generate retrovirus introduced with the IL-7×CCL19 expression vector (1) or the control vector (1). ..

    Plasmid Preparation:

    Article Title: Immunocompetent cell and expression vector expressing regulatory factors of immune function
    Article Snippet: Also, the anti-FITC CAR DNA fragment was cloned into the pMSGV retrovirus expression vector to generate a pMSGV vector free from IL-7 and CCL19 as a control (control vector (1)). (Generation of Retrovirus Introduced with IL-7×CCL19 Expression Vector) For the transduction of mouse T cells, retrovirus was generated. .. A GP2-293 packaging cell line (manufactured by Takara Bio Inc.) was transfected with the aforementioned IL-7×CCL19 expression vector (1) or control vector (1) and a pCL-Eco plasmid (manufactured by Imgenex Corp.) using Lipofectamine 2000 or 3000 (manufactured by Life Technologies Corp.) to generate retrovirus introduced with the IL-7×CCL19 expression vector (1) or the control vector (1). ..

    Control:

    Article Title: Immunocompetent cell and expression vector expressing regulatory factors of immune function
    Article Snippet: Also, the anti-FITC CAR DNA fragment was cloned into the pMSGV retrovirus expression vector to generate a pMSGV vector free from IL-7 and CCL19 as a control (control vector (1)). (Generation of Retrovirus Introduced with IL-7×CCL19 Expression Vector) For the transduction of mouse T cells, retrovirus was generated. .. A GP2-293 packaging cell line (manufactured by Takara Bio Inc.) was transfected with the aforementioned IL-7×CCL19 expression vector (1) or control vector (1) and a pCL-Eco plasmid (manufactured by Imgenex Corp.) using Lipofectamine 2000 or 3000 (manufactured by Life Technologies Corp.) to generate retrovirus introduced with the IL-7×CCL19 expression vector (1) or the control vector (1). ..

    other:

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: 3. cDNA, Clontech, Cat no. 637301, (Brain, Heart, day 7 Embrio, Testis, Spleen).

    Article Title: Anti-PVRIG antibodies and methods of use
    Article Snippet: 2. cDNA panel II, Mouse, Biochain, Cat no. C8334502 (Lung, Pancreas, Spleen, Skeletal Muscle).



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